3FastGeno FAQ
1， why I got no band?
a, check the RedMaster 2X PCR mix work or not?
add a purified gDNA and/or a taq polymerase as control
b, check primer
Use other primer as a control?
c, check your thermocycle setting
do you follow the recommend thermo protocol in our kit?
d, check your gDNA quantity and quality
add a purified gDNA as template control
e, Did you use the DE buffer extracted DNA together with the RedMaster 2X PCR mix in our kit?
please use all reagents in our kit together. We can not guarantee other Taq polymerase can amplify the DNA extracted with DE buffer.
2, I first use 3FastGeno kit. How decide my first PCR annealing tempreture?
a, Do gradient or Veriflex PCR to determine the best PCR annealing tempreture
b, The annealing temperature of PCR should be approximately 10°C higher than the primer Tm.
Calculate your primer Tm
3, How to get rid of nonspecific band?
a, please optimize your annealing teperature of your PCR with gradient PCR or Veriflex PCR.
b, add a No Template Control